Seminars 2013

Metabolomic Study of TiO2 and CeO2 Nanomaterials Effects in Human Liver HepG2 Cells

Kirk T. Kitchin, PhD., DABT

3:30 PM
Friday, February 15, 2013               
Room 125 Butler-Carlton Hall

Human liver HepG2 cells were exposed to four TiO2 and two CeO2 nanomaterials (either 3 or 30 ug/ml for three days) with dry sizes ranging from 6 to 410 nm. A metabolomics study was then performed using three mass spectroscopy dependent platforms (LC and GC). All but one of the nanomaterials strongly depressed reduced glutathione concentration. Two large decreases in glutathione (GSH) concentration (to 22% and 13% of control values) were from exposures to a CeO2 (8 nm) and a TiO2 (59 nm) both from Nanoamor, respectively. The decreases in the GSH system were observed both in (a) GSH precursors (glutamate and cysteine), (b) GSH itself and (c) GSH metabolites (the gamma-glutamyl condensation products with glutamate, glutamine, alanine, valine and also 5-oxoproline and cysteine-GSH). Other interesting effects noted in this metabolomics study were (a) increased asymmetric dimethylargine concentration and thus possible iNOS and NO concentration effects and (b) increased concentrations of many lipids particularly fatty acids. The observed glutathione depletion is consistent with nanomaterials acting via an oxidative stress mode of action in their toxic effects.

Practical Research and Engineering for the Real World

Robert Brownwood

3:30 PM
Friday, February 8, 2013               
Room 125 Butler-Carlton Hall

With money supplies shrinking and demand for financial resources multiplying the Engineering and Academic communities find themselves in a highly competitive market that will require an acute ability to work within the margins of industry to secure funding.  Just as hydraulic fracturing (fracking) changed the U.S. outlook on natural gas reserves, water utility partnerships can change the research landscape and provide unparalleled benefit to future generations.  Innovative methods to secure research funding will be discussed as well as examples of lost opportunities due to academia’s unwillingness to see the benefits of mining for the less “attractive” dollars.  This presentation will also discuss the practical implementation of engineering principals in the water industry. 

Mr. Brownwood graduated from California State University with a B.S. in Civil Engineering in 1991 and with an M.S. in Civil Engineering in 1996, both degrees emphasizing environmental and geotechnical engineering.  He obtained his California PE in 1996 and a T-5 Water Plant Operators certificate in 1997.

Mr. Brownwood has worked for the California Department of Public Health, Division of Drinking Water, from 1989 through 2004 inspecting public water systems throughout California to ensure compliance with the Safe Drinking Water Act and all appropriate engineering specifications.  He has been the Water Supply Manager for the City of Tulsa since 2004 and currently manages a staff of 105 employees, a $20,000,000 a year operating budget and over a billion dollars in assets consisting of two lakes and associated dams, 200 miles of large diameter transmission mains, eight pumping stations, four surface water treatment plants and multiple ancillary facilities. 

Lab Safety Training

Xinsheng Zhang

3:30 PM
Friday, February 1, 2013               
Room 125 Butler-Carlton Hall

The Missouri University of Science and Technology (Missouri S&T) is committed to providing a safe learning and working environment for our students and employees. As part of this commitment, lab safety training is required to establish a standard format for safety in the laboratories of the university.

In this seminar, we will discuss general lab safety rules, protective equipment, chemical hygiene plan, chemical storage, and chemical waste management. After training, you should be able to protect yourself in a lab. Your safety is our priority.